Crossed Immunoelectrophoresis Analysis of Bee Venom
- 1 May 1982
- Vol. 37 (4), 273-278
- https://doi.org/10.1111/j.1398-9995.1982.tb01910.x
Abstract
Bee venom obtained by electrical stimulation was analyzed by crossed immunoelectrophoresis against high-titer rabbit antibodies. The antigenic analysis of bee venom revealed that the extract contained 17 antigens, which were detected in the crossed immunoelectrophoresis pattern after staining with Coomassie Brilliant Blue. The crossed immunoelectrophoresis pattern indicated heterogeneity in several of the antigens, as most of the precipitates (exhibiting enzyme and hemolytic activities) represented multiple forms of phospholipase A, hyaluronidase, acid phosphatase and melittin.Keywords
This publication has 14 references indexed in Scilit:
- Isotachophoretic characterization of stinging insect venomsJournal of Chromatography A, 1981
- Application of high-speed aqueous gel permeation chromatography to insect venomsJournal of Chromatography A, 1980
- A high molecular weight allergenic fraction of honeybee venomJournal of Allergy and Clinical Immunology, 1977
- Allergens in bee venomJournal of Allergy and Clinical Immunology, 1977
- Melittin: An allergen of honeybee venomJournal of Allergy and Clinical Immunology, 1977
- Allergens in bee venom II. Two new high molecular weight allergenic specificitiesJournal of Allergy and Clinical Immunology, 1977
- Allergens in bee venom *1I. Separation and identification of the major allergensJournal of Allergy and Clinical Immunology, 1976
- Allergens of honey bee venomArchives of Biochemistry and Biophysics, 1976
- A sensitive and specific plate test for the quantitation of phospholipasesAnalytical Biochemistry, 1972
- Esterases and Phosphatases of Honeybee VenomJournal of Apicultural Research, 1967