Guanosine 5'‐triphosphate releases calcium from rat liver and guinea pig parotid gland endoplasmic reticulum independently of inositol 1,4,5‐trisphosphate
- 7 July 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 202 (2), 267-273
- https://doi.org/10.1016/0014-5793(86)80699-8
Abstract
GTP releases calcium from rat liver microsomes and guinea pig parotid gland microsomal subfractions independently of the presence of inositol 1,4,5-trisphosphate (IP3). Non-hydrolyzable guanine nucleotide analogues have no effect and inhibit the effect of GTP. The mechanism of GTP-mediated calcium release differs from IP3-mediated calcium release as indicated by the following findings: (i) GTP-induced calcium release depends on the presence of compounds which increase the viscosity of the medium (polyethylene glycol, polyvinylpyrrolidone, or bovine serum albumin); (ii) GTP-mediated calcium release is much slower; (iii) GTP-mediated calcium release is strongly temperature-dependent, whereas IP3-mediated calcium release is not; (iv) GTP-mediated calcium release is much more sensitive to a decrease of intravesicular free calcium than IP3-mediated calcium releaseKeywords
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