The influence of the Klebsiella pneumoniae regulatory gene nifL upon the transcriptional activator protein NifA
- 1 August 1990
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 4 (8), 1253-1258
- https://doi.org/10.1111/j.1365-2958.1990.tb00704.x
Abstract
The influence of the Klebsiella pneumoniae nifL gene product upon the interaction of the transcriptional activator protein NifA with the nifH promoter has been examined using in vivo dimethylsulphate ‘footprinting’. Binding of NifA to the upstream activator sequence (UAS) of the nifH promoter in the presence of the NifL protein was observed under nitrogen‐limiting growth conditions. Growth in the presence of NH+4 or addition of NH+4 to nitrogen‐limited cells diminished the interaction of NifA with the UAS when NifL was present. Repression of nif transcription by NifL may therefore involve an interaction between NifL and NifA which reduces the affinity of NifA for the UAS.This publication has 37 references indexed in Scilit:
- Characterisation of the Klebsiella pneumoniae nitrogen‐fixation regulatory proteins NIFA and NIFL in vitroEuropean Journal of Biochemistry, 1990
- In vivo studies on the interaction of RNA polymerase-σ54 with the Klebsiella pneumoniae and Rhizobium meliloti nifH promotersJournal of Molecular Biology, 1989
- A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation.Genes & Development, 1989
- Function of a Bacterial Activator Protein That Binds to Transcriptional EnhancersScience, 1989
- The role of activator binding sites in transcriptional control of the divergently transcribed nifF and nif LA promoters from Klebsiella pneumoniaeMolecular Microbiology, 1988
- Sequence of nifL from Klebsiella pneumoniae: mode of action and relationship to two families of regulatory proteinsMolecular Microbiology, 1987
- Conserved domains in bacterial regulatory proteins that respond to environmental stimuliCell, 1987
- Frameshifts close to the Klebsiella pneumoniae nifH promoter prevent multicopy inhibition by hybrid nifH plasmidsMolecular Genetics and Genomics, 1987
- Upstream activator sequences are present in the promoters of nitrogen fixation genesNature, 1986
- Nitrogen fixation gene (nifL) involved in oxygen regulation of nitrogenase synthesis in K. peumoniaeNature, 1981