Control of transcription initiation in vitro requires binding of a transcription factor to the distal promoter of the ovalbumin gene.
Open Access
- 1 August 1986
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 6 (8), 2784-2791
- https://doi.org/10.1128/mcb.6.8.2784
Abstract
We used a cell-free HeLa cell transcription system to identify and characterize transcription factors and the promoter elements that they recognize in RNA polymerase II-transcribed genes. Deletion of the region (-71 to -83) containing the GTCAAA direct repeat resulted in a marked decrease of specific transcription of the ovalbumin gene; transcription could be competed with DNA fragments containing this sequence. Furthermore, DNase I footprinting identified a protein-binding site including this direct repeat with crude extracts and one of the partially purified protein fractions required for transcription. We propose that a soluble factor activates transcription through binding to the direct repeat of GTCAAA sequence upstream from the ovalbumin gene.This publication has 46 references indexed in Scilit:
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