Abstract
Fe+++ added to acidulated whole blood is partly reduced, Fe++ is partly oxidized. These reactions do not take place when Fe++ or Fe+++ are added to plasma or serum. The ether used must contain peroxide. The "loosely combined" Fe of the blood was found highest in a horse with secondary anemia caused by repeated bleeding, the value returning to normal after a rest period. CO inhibits the ionization by acid of one of the Fe compounds, but neither the ionization of Fe++ nor of Fe+++ added to blood. It is impossible to recover quantitatively Fe immediately after its addition to the blood. The fraction which is at once soluble in acid consists of Fe+++ only.

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