Growth of Human Epidermoid Carcinomas (Strains KB and HeLa) in Hamsters from Tissue Culture Inocula.

Abstract

The KB and HeLa strains of human epidermoid carcinoma were established in the cheek pouches of golden hamsters by means of quantitated suspensions prepared from tissue cultures in a medium providing the specific amino acid and vitamin requirements of these cell lines. Strain KB, isolated directly from a biopsy specimen in these media, was grown in LAF1 mice bearing homologous ACTH secreting pituitary tumors. This strain was also established in the cheek pouch of non-conditioned hamsters but thus far has failed to grow in noncon-ditioned mice. Use of quantitated tissue culture inocula for the production of tumors in the hamster cheek pouch for chemo-therapeutic studies is suggested.