Substrate structural requirements of Schizosaccharomyces pombe RNase P
- 17 July 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 251 (1-2), 84-88
- https://doi.org/10.1016/0014-5793(89)81433-4
Abstract
RNase P from Schizosaccharomyces pombe has been purified over 2000-fold. The apparent K m for two S. pombe tRNA precursors derived from the supS1 and sup3-e tRNASer genes is 20 nM; the apparent V max is 2.5 nM/min (supS1) and 1.1 nM/min (sup3-e). Processing studies with precursors of other mutants show that the structures of the acceptor stem and anticodon/intron loop of tRNA are crucial for S. pombe RNase P action.Keywords
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