Dissociation of tissue inhibitor of metalloproteinases (TIMP) from enzyme complexes yields fully active inhibitor
- 1 August 1989
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 261 (3), 1031-1034
- https://doi.org/10.1042/bj2611031
Abstract
Recombinant human tissue inhibitor of metalloproteinases (TIMP) forms complexes with high-Mr active recombinant stromelysin that are stable over long periods under physiological conditions. TIMP-stromelysin complexes could be dissociated in the presence of EDTA at pH 3, releasing free TIMP and destroying stromelysin activity. The dissociated TIMP was apparently unmodified, in contrast with other known protein inhibitors of metalloproteinases and many classes of serine-proteinase inhibitor, which are slowly cleaved.This publication has 17 references indexed in Scilit:
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