Search for ribosomal mutants inPodospora anserina: genetic analysis of cold-sensitive mutants

Abstract

SUMMARY: Twenty-four cold-sensitive (prototrophic) mutants were isolated after UV mutagenesis of protoplasts of the fungusPodospora anserina. Genetic analysis of these mutants was performed in order to detect those among them which were most likely to be impaired in translational fidelity. The 24 mutations belonged to 24 different genes. One half of the mutants were pleiotropic and displayed an altered phenotype: growth rate at the permissive temperature, germination of the spores, fertility and/or sporulation. Nine mutants differed from wild-type in their resistance levels to cycloheximide, trichodermin and/or paromomycin. Several mutations were linked to known ribosomal loci. Two mutations behaved like informational antisuppressors: one is allelic to the previously describedAs₃gene and the other one defines a new antisuppressor gene,AS₆.