Demonstration of clonal variation amongst O-antigen serotype variants of Escherichia coli O2 and O18 using DNA probes to the rfb region of the E. coli strain B41 (O101 : K99/F41)

Abstract

The confirmation at the DNA level of the existence of clonal variants within Escherichia coli O2 and O18 serotypes has been shown by Southern hybridization analysis of restriction endonuclease digested genomic DNA and subsequent probing with contiguous subclones of the E. coli O101 rfb region. The O101 rfb subclones are believed to represent a conserved region of DNA (Heuzenroeder et al. Molec. Microbiol, in press) and identify serotype variants by means of restriction fragment length polymorphisms (RFLP) within homologous DNA of O2 and O18 E. coli. A number of different restriction enzymes have been used singly and in combination to digest the genomic DNA, thereby allowing construction of restriction maps of the region displaying homology to the O101 rfb region subclones. This analysis further substantiates previously defined evolutionary relationships between O2 and O18 E. coli. These simple probes appear to be able to provide the same clonal information as a battery of isoenzyme, outer membrane protein (OMP) and lipopolysaccharide (LPS) analyses.