STUDIES ON THE MECHANISM OF ACETATE OXIDATION BY BACTERIA
Open Access
- 20 July 1951
- journal article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 34 (6), 785-794
- https://doi.org/10.1085/jgp.34.6.785
Abstract
1. Simultaneous oxidation of C14-methyl-labeled acetate, and unlabeled malate or fumarate and α-ketoglutarate results in entrapment of labeled carbon in the C4-dicarboxylic acids, but not in α-ketoglutarate, although all substrates are utilized at comparable rates. 2. A large endogenous reduction of all C4-dicarboxylic acids (fumarate, oxalacetate, and malate) to succinate is observed under aerobic conditions, and when vigorous oxidation is proceeding. This effect occurs with both freshly harvested young (18 hour) cells and stored (2 week) cells. 3. This reduction can be considerably minimized under high oxygen tensions. 4. The quantitative concordance of these results with a Thunberg-Knoop cyclic mechanism for acetate oxidation is shown. Possible alternative C4 products formed prior to succinate are not completely excluded, but it appears that the cells can utilize the succinate condensation as a major pathway in acetate oxidation.Keywords
This publication has 3 references indexed in Scilit:
- STUDIES ON THE MECHANISM OF ACETATE OXIDATION BY ESCHERICHIA COLIJournal of Biological Chemistry, 1951
- PRODUCTS OF THE OXIDATION OF GLYCOLIC ACID AND l-LACTIC ACID BY ENZYMES FROM TOBACCO LEAVESJournal of Biological Chemistry, 1949
- The role of fumarate in the respiration of Bacterium coli communeBiochemical Journal, 1937