IODINE METABOLISM IN SURVIVING HUMAN THYROID SLICES MAINTAINED IN A MEDIUM OF CONSTANT COMPOSITION1

Abstract

IN A previous paper (Molnar et al., 1956) it has been shown that rates of I¹³¹ uptake by surviving human and canine thyroid slices could not be determined by the method of incubation of slices in a fixed volume of medium containing I¹³¹. A new method was therefore devised in which a medium of constant composition flowed by gravity at a constant rate over thyroid slices. This method, termed “gravity-flow incubation,” and its potentialities and limitations form the subject of this paper.