The effects of anti‐CD2 antibodies on the differentiation of mouse thymocytes
- 1 May 1989
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 19 (5), 951-954
- https://doi.org/10.1002/eji.1830190526
Abstract
Using a rat monoclonal antibody against mouse CD2, we determined the expression of this marker on thymocytes during ontogeny. CD2 expression becomes detectable at day 15 and reaches adult levels (∼95% positivity) by day 19. Furthermore, the effect of anti‐CD2 antibodies on T cell differentiation was analyzed by addition of antibodies to thymus organ cultures or repeated injection into newborn mice. Anti‐CD2 antibodies inhibit CD2 expression in organ cultures and drastically reduce its expression on thymocytes and peripheral lymphocytes in vivo. In either situation, suppression of CD2 expression does not significantly alter the generation of T cells expressing CD3, CD4, CD8 and T cell receptor Vβ8. These results do not support a role for CD2 in early steps of thymocyte differentiation.This publication has 22 references indexed in Scilit:
- Antibodies against the T cell receptor/CD3 complex interfere with distinct intra-thymic cell-cell interactionsin vivo: correlation with arrest of T cell differentiationEuropean Journal of Immunology, 1989
- Deletion of self-reactive T cells before entry into the thymus medullaNature, 1988
- Positive and negative selection of an antigen receptor on T cells in transgenic miceNature, 1988
- The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigensNature, 1988
- Positive selection of antigen-specific T cells in thymus by restricting MHC moleculesNature, 1988
- Early events in human T cell ontogeny. Phenotypic characterization and immunohistologic localization of T cell precursors in early human fetal tissues.The Journal of Experimental Medicine, 1988
- The Lymphocyte Function Associated LFA-1, CD2, and LFA-3 Molecules: Cell Adhesion Receptors of the Immune SystemAnnual Review of Immunology, 1987
- Expression of antigen-specific, major histocompatibility complex-restricted receptors by cortical and medullary thymocytes in situCell, 1985
- A molecular basis for thymic selection: regulation of T11 induced thymocyte expansion by the T3-Ti antigen/MHC receptor pathwayImmunology Today, 1985
- Effect of deoxyguanosine on lymphopoiesis in the developing thymus rudiment in vitro: Application in the production of chimeric thymus rudimentsEuropean Journal of Immunology, 1982