Presence of T145 on cytolytic T cell lines and their lectin‐resistant mutants

Abstract

Murine cytolytic T cell lines have been analyzed for the expression of two surface glycoproteins called T 145 and T 130. T 145, known to be expressed by activated cytolytic T cells, is also expressed by such lines, but T 130, which has been described as a universal T cell marker, is not. Our results suggest a structural relationship between T 145 and T 130. Vicia villosa lectin, which binds selectively to T 145 of activated T cells and which is cytotoxic for cytolytic T cell lines, has been used to select lectin-resistant mutants from these lines. Five independent lectin-resistant mutants have been obtained. All of them are cytolytically active, bind up to 100-fold less lectin than the parental lines, but still express T 145 or a closely related glycoprotein.