The mechanism of anaphylaxis: observations on the failure of antibodies from certain species to sensitize guinea pigs in direct and reversed passive anaphylaxis.

Abstract

Using the criteria of generalized anaphylaxis, the Schultz-Dale test, and mast cell degranulation occurring on contact with antigen in vitro, a study has been made of the ability of antisera, prepared in various species, to sensitize guinea pig tissues passively. Whereas small amounts of guinea pig and rabbit antibodies and rather larger amounts of monkey, dog and human antibodies were effective, antibodies from goat, horse, rat, fowl and a human auto-immune thyroid antiserum were ineffective. These results are in general agreement with those in the literature.