Gaucher's disease Factors affecting the 4-methylum-belliferyl-β-D-glucosidase activity of cultured skin fibroblasts
- 23 April 2008
- journal article
- research article
- Published by Wiley in Clinical Genetics
- Vol. 14 (2), 77-79
- https://doi.org/10.1111/j.1399-0004.1978.tb02109.x
Abstract
Phosphate-buffered saline inhibited acid .beta.-glucosidase activity of cultured cells. This effect was attributable to both the pH and the chloride content of the phosphate-buffered saline. Washing the cells in sucrose (0.25 M) to remove residual phosphate-buffered saline effectively eliminated this inhibition.Keywords
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