Characterization of pyrimidine deoxyribonucleoside kinase (thymidine kinase) and thymidylate kinase as a multifunctional enzyme in cells transformed by herpes simplex virus type 1 and in cells infected with mutant strains of herpes simplex virus
- 1 June 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 30 (3), 942-945
- https://doi.org/10.1128/jvi.30.3.942-945.1979
Abstract
Pyrimidine deoxyribonucleoside kinase (thymidine kinase [TK]) was purified from 2 herpes simplex virus type 1 (HSV-1)-transformed TK-deficient mouse (LMTK-) cell lines and from LMTK- cells infected with HSV-1 mutant viruses coding for variant TK enzymes. These preparations exhibited normal or variant virus-induced thymidylate kinase activities correlating with their relative TK activities. Neither virus-induced activity was detected in LMTK- cells infected with an HSV-1 TK-deficient mutant. HSV-1 thymidylate kinase activity and TK activity are mediated by the same protein.This publication has 12 references indexed in Scilit:
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