Comparison of Murine B Cell Clonal Expansions by Synthetic Lipid A and Muramyl Dipeptide Analogs
- 14 November 1985
- journal article
- research article
- Published by Wiley in Microbiology and Immunology
- Vol. 29 (11), 1111-1120
- https://doi.org/10.1111/j.1348-0421.1985.tb00901.x
Abstract
Direct stimulations of murine B lymphocytes with synthetic lipid A analogs and synthetic muramyl dipeptide (MDP) derivatives were studied using a limiting dilution assay system. Synthetic lipid A analogs, GLA-27 and GLA-40, when conjugated with bovine serum albumin (BSA) had the ability to induce B cell clonal expansion of a single B cell from the spleen or bone-marrow. Their activities were almost the same as those of naturally obtained lipid A, but were lower than that of bacterial lipopolysaccharide (LPS). Addition of dextran sulfate (DSX) enhanced the effect of lipid A analogs. In contrast, synthetic MDP and its derivatives, although they had many biological and immunological activities in experimental animals, could not stimulate a single B cell to induce clonal expansion regardless of the presence or absence of DXS. These results suggested that lipid A analogs can directly cause the proliferation of B cells, but MDPs can not.This publication has 62 references indexed in Scilit:
- Biological activities of analogues of lipid A based chemically on the revised structural modelEuropean Journal of Biochemistry, 1984
- Endotoxic properties of chemically synthesized lipid A part structures. Comparison of synthetic lipid A precursor and synthetic analogues with biosynthetic lipid A precursor and free lipid AEuropean Journal of Biochemistry, 1984
- Biological activities of chemically synthesized analogues of the nonreducing sugar moiety of lipid AFEBS Letters, 1984
- Mitogenic and polyclonal B cell activation activities of synthetic lipid A analoguesEuropean Journal of Immunology, 1984
- Interferon‐inducing, pyrogenic and proclotting enzyme of horseshoe crab activation activities of chemically synthesized lipid A analoguesEuropean Journal of Biochemistry, 1983
- Biological Activity of Chemically Synthesized Analogues of Lipid AEuropean Journal of Biochemistry, 1982
- Synergism of bacterial lipopolysaccharides and concanavalin A in the activation of thymic lymphocytesCellular Immunology, 1975
- Minimal structural requirements for adjuvant activity of bacterial peptidoglycan derivativesBiochemical and Biophysical Research Communications, 1974
- Selective induction of DNA synthesis in T and B lymphocytesCellular Immunology, 1972
- A QUANTITATIVE ASSAY FOR THE PROGENITORS OF BONE MARROW-ASSOCIATED LYMPHOCYTESThe Journal of Experimental Medicine, 1972