Propagation of adipogenic signals through an epigenomic transition state
- 15 May 2010
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 24 (10), 1035-1044
- https://doi.org/10.1101/gad.1907110
Abstract
The transcriptional mechanisms by which temporary exposure to developmental signals instigates adipocyte differentiation are unknown. During early adipogenesis, we find transient enrichment of the glucocorticoid receptor (GR), CCAAT/enhancer-binding protein β (CEBPβ), p300, mediator subunit 1, and histone H3 acetylation near genes involved in cell proliferation, development, and differentiation, including the gene encoding the master regulator of adipocyte differentiation, peroxisome proliferator-activated receptor γ2 (PPARγ2). Occupancy and enhancer function are triggered by adipogenic signals, and diminish upon their removal. GR, which is important for adipogenesis but need not be active in the mature adipocyte, functions transiently with other enhancer proteins to propagate a new program of gene expression that includes induction of PPARγ2, thereby providing a memory of the earlier adipogenic signal. Thus, the conversion of preadipocyte to adipocyte involves the formation of an epigenomic transition state that is not observed in cells at the beginning or end of the differentiation process.Keywords
This publication has 44 references indexed in Scilit:
- PPARγ and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scaleGenes & Development, 2008
- Glucocorticoid Signaling Defines a Novel Commitment State during AdipogenesisIn VitroMolecular Biology of the Cell, 2008
- PRDM16 controls a brown fat/skeletal muscle switchNature, 2008
- Combinatorial patterns of histone acetylations and methylations in the human genomeNature Genetics, 2008
- DOT1L/KMT4 Recruitment and H3K79 Methylation Are Ubiquitously Coupled with Gene Transcription in Mammalian CellsMolecular and Cellular Biology, 2008
- FoxA1 Translates Epigenetic Signatures into Enhancer-Driven Lineage-Specific TranscriptionCell, 2008
- Asap: A Framework for Over-Representation Statistics for Transcription Factor Binding SitesPLOS ONE, 2008
- Network motifs: theory and experimental approachesNature Reviews Genetics, 2007
- Distinct and predictive chromatin signatures of transcriptional promoters and enhancers in the human genomeNature Genetics, 2007
- The Mammalian EpigenomeCell, 2007