Molecular cloning and characterization of two novel transport proteins from rat kidney
Open Access
- 20 March 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 425 (1), 79-86
- https://doi.org/10.1016/s0014-5793(98)00203-8
Abstract
The recent cloning of renal transport systems for organic anions and cations (OAT1, OCT1, and OCT2) opened the possibility to search, via polymerase chain reaction (PCR) homology screening, for novel transport proteins. Two integral membrane proteins, UST1 and UST2, were cloned from rat kidney. RT‐PCR revealed that UST1 is confined to the kidney whereas UST2 mRNA was detected in all tested tissues. Sequence analyses suggest that UST1 and UST2, together with four related transporters, comprise, within the major facilitator superfamily, a so far unrecognized transporter family, termed amphiphilic solute facilitator (ASF) family. Characteristic signatures for the ASF family were identified.Keywords
This publication has 26 references indexed in Scilit:
- Molecular Cloning and Characterization of NKT, a Gene Product Related to the Organic Cation Transporter Family That Is Almost Exclusively Expressed in the KidneyPublished by Elsevier ,1997
- Quartet Puzzling: A Quartet Maximum-Likelihood Method for Reconstructing Tree TopologiesMolecular Biology and Evolution, 1996
- cDNA Cloning and Functional Expression of a Novel Rat Kidney Organic Cation Transporter, OCT2Biochemical and Biophysical Research Communications, 1996
- SCOP: A structural classification of proteins database for the investigation of sequences and structuresJournal of Molecular Biology, 1995
- Structural features of the uniporter/symporter/antiporter superfamilyProtein Science, 1995
- A major superfamily of transmembrane facilitators that catalyse uniport, symport and antiportTrends in Biochemical Sciences, 1993
- The rapid generation of mutation data matrices from protein sequencesBioinformatics, 1992
- Basic local alignment search toolJournal of Molecular Biology, 1990
- PHYLOGENIES FROM MOLECULAR SEQUENCES: INFERENCE AND RELIABILITYAnnual Review of Genetics, 1988
- Sequence and Structure of a Human Glucose TransporterScience, 1985