An improved separation procedure for nucleoside monophosphates on polyethyleneimine- (PEI-)cellulose thin layers

Abstract
A procedure is described for the two-dimensional separation of the 4 major and 16 modified nucleoside-(5′) monophosphates on anion-exchange thin layers of polyethyleneimine-(PEI-)cellulose. The method, which is simple and less time-consuming than existing partition chromatographic methods, may be used for the identification of 5′-termini of RNA and RNA fragments.

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