An improved separation procedure for nucleoside monophosphates on polyethyleneimine- (PEI-)cellulose thin layers
- 1 November 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (11), 2915-2922
- https://doi.org/10.1093/nar/3.11.2915
Abstract
A procedure is described for the two-dimensional separation of the 4 major and 16 modified nucleoside-(5′) monophosphates on anion-exchange thin layers of polyethyleneimine-(PEI-)cellulose. The method, which is simple and less time-consuming than existing partition chromatographic methods, may be used for the identification of 5′-termini of RNA and RNA fragments.Keywords
This publication has 13 references indexed in Scilit:
- Sequence studies on tRNAPhe from human placenta: Comparison with known sequences of tRNAPhe from other normal mammalian tissuesBiochemical and Biophysical Research Communications, 1975
- Structural analysis of nonradioactive rna by postlabeling: The primary structure of baker's yeast tRNACUALeuBiochemical and Biophysical Research Communications, 1975
- Sequence analysis of nonradioactive RNA fragments by periodate-phosphatase digestion and chemical tritium labeling: characterization of large oligonucleotides and oligonucleotides containing modified nucleosidesNucleic Acids Research, 1974
- The corrected nucleotide sequence of yeast leucine transfer ribonucleic acidBiochemical and Biophysical Research Communications, 1973
- Absence of the Sequence G-T-Ψ-C-G(A)- in Several Eukaryotic Cytoplasmic Initiator Transfer RNAsProceedings of the National Academy of Sciences, 1973
- Minor components in transfer RNA: their characterization, location, and function.1972
- Transfer Ribonucleic Acid from Mycoplams laidlawii AEuropean Journal of Biochemistry, 1971
- Structure of the fluorescent Y base from yeast phenylalanine transfer ribonucleic acidJournal of the American Chemical Society, 1970
- Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- A nuclease specific for heat-denatured DNA isolated from a product of Aspergillus oryzaeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966