Reactivity of Hybridoma Antibodies with Amniotic and Plasma Fibronectin

Abstract
Mouse antibodies were prepared to human amniotic fluid fibronectin by somatic cell hybrids [mouse myeloma MOPC 21 cell spleen cell hybridoma]. Comparison of immunological reactivity of antibodies with amniotic and plasma fibronectin revealed that the 2 molecules had very similar patterns of antigenic determinants. Antibodies from 1 cell population bound better to amniotic fluid fibronectin, which indicates that there is a difference in the molecular structure of fibronectins isolated from the 2 sources.