Detection and Isolation of a New DNA Polymerase from Human Breast Tumor Cell Line HBT-3 by (dT) 12-18 -Cellulose Chromatography

Abstract

Column chromatography on (dT)(12-18)-cellulose was used to detect and isolate a novel DNA polymerase activity from human breast tumor cell line HBT-3. The new enzyme is similar to those of mammalian RNA tumor viruses in its molecular size, preference for poly(A).(dT)(12-18) as compared to either poly(dA).(dT)(12-18) or poly(A).poly(dT), and ability to copy poly(C).(dG)(12-18). A similar DNA polymerase activity, defined by its binding to (dT)(12-18)-cellulose, is not detectable in several other cell lines of human tumors.