Endothelium-dependent contractions to arachidonic acid are mediated by products of cyclooxygenase
- 1 April 1985
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 248 (4), H432-H437
- https://doi.org/10.1152/ajpheart.1985.248.4.h432
Abstract
Arachidonic acid produces endothelium-dependent relaxation in canine arteries and endothelium-dependent contraction in veins. In canine femoral arteries, the relaxation is prevented by inhibitors of cyclooxygenase. To determine the role of cyclooxygenase in the contraction evoked by arachidonic acid in the veins, rings of canine femoral and intrapulmonary veins, with and without endothelium, were suspended in organ chambers and set at their optimum length for isometric tension measurements. In rings of femoral and pulmonary vein contracted with norepinephrine, arachidonic acid produced a concentration-dependent increase in tension that was eliminated by removal of the endothelium or by treatment with the inhibitors of cyclooxygenase (indomethacin, meclofenamate, or acetylsalicyclic acid). The contractions were not prevented by inhibitors of thromboxane synthetase or prostacyclin synthetase or lipoxygenase. Pulmonary and femoral veins with or without endothelium relaxed to low, but contracted to high concentrations of prostacyclin and prostaglandin E2. Prostaglandin F2 alpha caused endothelium-independent contractions in both blood vessels. The present study suggests that the endothelium-dependent contractions to arachidonic acid observed in canine veins are mediated by prostanoids other than thromboxane and prostacyclin.This publication has 17 references indexed in Scilit:
- Slow-reacting substances (leukotrienes) contract human airway and pulmonary vascular smooth muscle in vitroNature, 1981
- The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholineNature, 1980
- Human Pulmonary Endothelial Cells in CultureJCI Insight, 1980
- PROSTACYCLIN AND THROMBOXANE-A2 SYNTHESIS BY RABBIT PULMONARY-ARTERY1980
- STIMULATION OF PROSTACYCLIN RELEASE FROM VESSEL WALL BY BAY g 6575, AN ANTITHROMBOTIC COMPOUNDThe Lancet, 1979
- CONTRACTILE RESPONSE OF ISOLATED RABBIT AORTIC STRIPS TO UNSATURATED FATTY-ACID PEROXIDES1979
- Contractile responses to prostacyclin (PGI2) of isolated human saphenous and rat venous tissueProstaglandins, 1978
- PGI2 production by rat blood vessels: Diminished prostacyclin formation in veins compared to arteriesProstaglandins, 1978
- The effect of prostacyclin on the human umbilical arteryProstaglandins, 1978
- Prostacyclin (PGI2) is a weak contractor of coronary arteries of the pigEuropean Journal of Pharmacology, 1977