Quantitative influence of antibody and complement coating of red cells on monocyte-mediated cell lysis.
Open Access
- 1 May 1978
- journal article
- research article
- Published by American Society for Clinical Investigation in JCI Insight
- Vol. 61 (5), 1309-1319
- https://doi.org/10.1172/jci109048
Abstract
Monocyte-mediated lysis in vitro of human red cells coated with measured amounts of immunoglobulin G (IgG) or complement were studied. 1,000-1,500 molecules of IgG anti-D are necessary to effect measurable lysis, and lysis increases linearly with increasing levels of antibody sensitization. 100 microgram/ml of IgG1 abolished lysis even at maximal levels of anti-D sensitization (15,000 molecules/cell). Two isoimmune IgG anti-A or anti-B antisera were 5 to 10-fold less efficient in promoting phagocytosis or lysis per molecule of IgG bound; however, because of the greater antigen density of A or B, more than 100,000 molecules IgG/cell could be bound, producing equivalent lysis to anti-D-coated cells. Although inhibition by IgG1 was similar at equivalent levels of sensitization with anti-A, anti-B, or anti-D at high levels of coating with anti-A or anti-B (not attainable with anti-D), lysis was not inhibited by IgG1. Cells coated with human complement components alone were not lysed by monocytes; however, complement coating augmented IgG-mediated lysis and reduced the quantity of anti-D necessary to produce lysis to less than 1,000 molecules/cell. After thorough degradation of C3b by serum to C3d, complement augmentation persisted.This publication has 20 references indexed in Scilit:
- Immunologic mechanisms in autoimmune hemolytic disease.1976
- Coombs--negative immune hemolytic anemia.1976
- MONOCYTE-MEDIATED ANTIBODY-DEPENDENT CELLULAR CYTOTOXICITY - CLINICAL TEST OF MONOCYTE FUNCTION1976
- Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation.The Journal of Experimental Medicine, 1975
- Specificity of human lymphocyte complement receptors.The Journal of Experimental Medicine, 1975
- TWO DIFFERENT COMPLEMENT RECEPTORS ON HUMAN LYMPHOCYTESThe Journal of Experimental Medicine, 1973
- Haemolytic activity of human blood monocytes. Lysis of human erythrocytes treated with anti-A serum.1973
- Quantitative immunology of immune hemolytic anemiaJCI Insight, 1971
- Human Monocytes: Distinct Receptor Sites for the Third Component of Complement and for Immunoglobulin GScience, 1968
- Red Cells Coated with Immunoglobulin G: Binding and Sphering by Mononuclear Cells in ManScience, 1967