Isolation and Characterization of An Immunosuppressive Protein From Trichosanthes Kirilowii Root Tubers
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Immunopharmacology and Immunotoxicology
- Vol. 9 (1), 25-46
- https://doi.org/10.3109/08923978709035200
Abstract
An immunosuppressive protein was isolated from Trichosanthes kirilowii root tubers by a procedure involving acetone fractionation and ion exchange chromatography on CM-Sepharose. Homogeneity of the protein was demonstrated in immunoelectrophoresis, SDS-polyacrylamide gel electrophoresis, gel filtration, high performance liquid chromatography and a single NH2-terminal sequence. The protein had a molecular weight of 26,000, aspartic acid as the NH2-terminal amino acid and no cysteine or carbohydrates in its molecule. It inhibited ConA-induced transformation in lymphocytes isolated from spleens of CBA mice. The protein was also potent in inducing mid-term abortion in mice.This publication has 8 references indexed in Scilit:
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