Additional Precipitation Reactions of Lectins with Human Serum Glycoproteins
- 1 January 1978
- journal article
- research article
- Published by Walter de Gruyter GmbH in cclm
- Vol. 16 (1), 19-24
- https://doi.org/10.1515/cclm.1978.16.1.19
Abstract
Highly purified human serum glycoproteins were treated with neuraminidase and examined for their cross reaction with several lectins with anti-galactosyl specificity: .beta.-D-galactosyl structures are apparently the main terminal sugar residues that become attached de novo after removal of neuraminic acid. The following lectins were tested: tridacnin from the bivalve clams Tridacna maxima and Tridacna gigas, the agglutinin from the sponge Axinella polypoides, the lectin from the roach Rutilus rutilus and the plant lectins from Ricinus communis, Ononis spinosa, Glycine soja and Abrus precatorius. In agar gel diffusion these purified and precipitating lectins gave more or less strong or negative results against the different neuroaminidase-treated serum glycoproteins, indicating subtle differences with respect to their anti-galactosyl combining specificity. Serum glycoproteins which reacted with the same lectin did not always show complete identity lines. As revealed by these lectins, the carbohydrate moiety of serum glycoproteins may reflect a complex and broad spectrum of heterogeneity. This could lead to a more detailed understanding of the topographical and steric arrangement of the chemical structure and the biological role of carbohydrate groups in these glycosubstances.Keywords
This publication has 14 references indexed in Scilit:
- The purification of porcine haptoglobin by affinity chromatography with concanavalin A – SepharoseCanadian Journal of Biochemistry, 1976
- Purification and characterization of the agglutinins from the sponge Axinella polypoides and a study of their combining sitesBiochemistry, 1976
- Comparative Aspects of Glycoprotein StructureAnnual Review of Biochemistry, 1976
- Similar combining specificities of invertebrate precipitins and mouse myeloma protein J 539 for β-(1→6)-galactansImmunochemistry, 1976
- Recent data on the structure of the carbohydrate moiety of glycoproteins. Metabolic and biological implicationsPublished by Walter de Gruyter GmbH ,1975
- Die pathogenetische Bedeutung mikrobieller NeuraminidasenDeutsche Medizinische Wochenschrift (1946), 1974
- [36] O-Glycosyl polyacrylamide gels: Application to phytohemagglutininsMethods in Enzymology, 1974
- Chemistry and Significance of the Carbohydrate Moieties of Human Serum GlycoproteinsAngewandte Chemie International Edition in English, 1973
- Concanavalin A Reaction with Human Normal Immunoglobulin G and Myeloma Immunoglobulin GScience, 1967
- Specific precipitins for type XIV pneumococcus polysaccharide fromAbrus precatorius seedsCellular and Molecular Life Sciences, 1961