Effect of Certain Compounds on in vitro Degradation of Serotonin by Ceruloplasmin.

Abstract

In an in vitro test system at pH 7.4 degradation of serotonin by ceruloplasmin was markedly inhibited by sodium metabisulfite, ascorbic acid, glutathione, BAL, isoniazid, iproniazid, hydralazine, an analog of hydralazine (Ba-20523), isocarboxazid, thiocarbohydrazide, hydrazine hydrate, syrosingopine (Su-3118) and its isomer, Su-5171. Lesser effects were produced by reserpine, tetraethylammonium chloride, and a thiazide diuretic (Su-7075). A number of other sulfhydryl containing chelating, anti-hypertension, and psychopharmacologic agents were without effect in the test system employed. It was not possible to correlate reaction inhibition with simple presence of sulfhydryl groups, the hydrazine nucleus, or with monoamine oxidase and diamine oxidase inhibition.