The src gene product of transformed and morphologically reverted ASV-infected mammalian cells

Abstract

Morphological revertants of avian sarcoma virus transformed vole cells contain the sarcoma gene product (pp60^src) in an enzymatically active form, suggesting that the presence of pp60^src protein kinase activity is insufficient to induce morphological transformation. Structural analyses of pp60^src from infected vole cell clones suggest that in one of the revertant clones an alteration in pp60^src may be responsible for morphological reversion while in a second clone, reversion may result from an alteration in a cell gene product with which pp60^src must interact. As these morphological revertant cells are tumorigenic, different cell components are required to interact with pp60^src to facilitate the two events.