An interface chip connection between capillary electrophoresis and thermal lens microscope
- 10 January 2003
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 24 (1-2), 179-184
- https://doi.org/10.1002/elps.200390011
Abstract
A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8×10-7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4×10-8 M, which was 100 times lower than that of conventional absorbance detection.Keywords
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