Nitrate Utilization by Nitrate Reductase-deficient Barley Mutants
- 1 April 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 67 (4), 740-743
- https://doi.org/10.1104/pp.67.4.740
Abstract
Two nitrate reductase-deficient barley mutants were studied for growth on nitrate and ammonium sources of nitrogen and for resistance to chlorate. Although nitrate reductase-deficient mutants in some species are chlorate-resistant (unable to reduce chlorate to chlorite), the barley mutants used in these studies when grown on nitrate and treated with chlorate were only slightly more resistant to chlorate than the control. When grown to maturity on vermiculite supplemented with either nitrate or ammonium nutrient solutions, the mutants produced as much dry weight and reduced nitrogen per plant as the control. The in vivo and in vitro nitrate reductase activities in the roots and shoots of the mutants grown on nitrate were consistently less than 10% of the control. To avoid the possibility that the mutants received reduced nitrogen from microbial sources, excised embryos were cultured under sterile conditions. Again the mutants were capable of growth and reduced nitrogen accumulation with nitrate as the sole source of nitrogen. In spite of the low apparent nitrate reductase activity, the nitrate reductase-deficient mutants are capable of substantial nitrate reduction.This publication has 12 references indexed in Scilit:
- Nitrate Assimilation in FungiPublished by Elsevier ,1979
- Induction and selection of specific gene mutations in hordeum and pisumMutation Research, 1978
- Effect of Herbicides on In Vivo Nitrate and Nitrite ReductionWeed Science, 1977
- Comparison of in Vivo and in Vitro Assays of Nitrate Reductase in Wheat (Triticum aestivum L.) SeedlingsPlant Physiology, 1976
- Chlorate toxicity in Aspergillus nidulansMolecular Genetics and Genomics, 1976
- Escherichia coli strain for use in nitrate analysisJournal of Agricultural and Food Chemistry, 1975
- Improvements of the Nitrite Color Development in Assays of Nitrate Reductase by Phenazine Methosulfate and Zinc AcetatePlant Physiology, 1974
- Use of Protein in Extraction and Stabilization of Nitrate ReductasePlant Physiology, 1974
- Nitrate reduction by higher plant peroxidaseFEBS Letters, 1973
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962