A Rapid Immunostaining Method Utilizing Preformed Antibody–Avidin–Biotin–Peroxidase Complexes

Abstract

A rapid immunostaining method utilizing a preformed antibody–avidin–biotin–peroxidase complex that produces staining in paraffin sections and in frozen sections of tissues in 15 minutes is described. Although the complex did not produce staining with the common leukocyte monoclonal antibody, consistent staining was seen with most antibodies used. The complex gave consistent and reproducible staining for at least three months when maintained in storage at 4 °C. The results show that many available antibodies can be adapted to a rapid one step staining procedure employing the avidin–biotin–peroxidase complex system.