Detection of Antigenically Distinctive Components of Mouse Melanoma S912

Abstract

Antiserums were produced in White Rock and New Hampshire Red cockerels against the S91 mouse melanoma and against tissues of normal mice of the inbred tumor-host strain (DBA/1). After absorption with normal mouse-tissue antigens, antiserums against the neoplasm showed significant precipitation titers of specific antibodies in reaction with the tumor antigen. Likewise, antiserums against normal mouse tissues precipitated normal tissue antigens after absorption with tumor antigen. It is clear, therefore, that the S91 melanoma and normal tissues of the host mouse strain possess distinctive antigenic components. In preliminary tests of a tumor of more recent origin, the B16 melanoma, similar antigenic differences between the neoplasm and tissues of its C57BL/6 host strain were observed.