Changes in chemical composition of chick embryos treated with a β-xyloside and a lathyrogen
- 1 June 1979
- journal article
- research article
- Published by Wiley in Teratology
- Vol. 19 (3), 345-356
- https://doi.org/10.1002/tera.1420190311
Abstract
Nine‐day chick embryos were treated with 4‐methylum‐belliferyl β‐D‐xyloside or β‐aminopropionitrile fumarate, and their gross chemical composition was examined one week later. Total DNA was 10–20% less in embryos treated with either drug than it was in control embryos. Xyloside‐treated embryos showed marked increases in percent wet weight and in sodium/DNA and chloride/DNA ratios, and small decreases in protein/DNA, hydroxyproline/DNA and sulfate/DNA. None of these parameters was affected in embryos treated with β‐aminopropionitrile. Approximately 85% of the uronic acid of control embryos was present as chondroitin sulfate, with a degree of sulfation of 80% and charge density of 1.8; all of this chondroitin sulfate was covalently linked to peptide and had a number‐average molecular weight of 29,300. In embryos treated with β‐xyloside, 90% of the uronic acid was present as chondroitin sulfate, with a degree of sulfation of 40% and charge density ranging from 1 to 2; 27% of this chondroitin sulfate, with an average molecular weight of 25,400, was peptide linked, while 73% was linked to 4‐methylum‐belliferone arid had an average molecular weight of 22,900. The chemical differences between embryos treated with the xyloside and embryos treated with the lathyrogen reinforce the conclusion on morphological grounds that these are distinct syndromes involving different aspects of the extracellular matrix.Keywords
This publication has 31 references indexed in Scilit:
- The mode of action of 4-methylumbelliferyl β-d-xyloside on the synthesis of chondroitin sulphate in embryonic-chicken sternumBiochemical Journal, 1977
- The micro-assay of the disaccharide isomers of chondroitin sulphateBiochimica et Biophysica Acta (BBA) - General Subjects, 1977
- The effect of β-d-xylosides on chondroitin sulphate biosynthesis in embryonic chicken cartilage in the absence of protein synthesis inhibitorsBiochemical Journal, 1977
- Alterations in the Metabolism of Glycosaminoglycans and CollagenPublished by Springer Nature ,1977
- The ubiquitous occurrence of chondroitin sulfates in chick embryosJournal of Experimental Zoology, 1975
- Synthesis of a fluorogenic mucopolysaccharide by chondrocytes in cell culture with 4-methylumbelliferyl β-d-xylosideBiochimica et Biophysica Acta (BBA) - General Subjects, 1975
- The molecular-weight distribution of glycosaminoglycansBiochemical Journal, 1973
- The quantitative measurement of Alcian Blue–glycosaminoglycan complexesBiochemical Journal, 1973
- Cross-linking of collagen and elastin. Properties of lysyl oxidaseBiochemistry, 1970
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956