SPECTROPHOTOMETRIC EXAMINATION OF HYDROGENASE AND NITROGENASE IN SOYBEAN NODULES AND Azotobacter
- 15 April 1957
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 43 (4), 297-304
- https://doi.org/10.1073/pnas.43.4.297
Abstract
Sonic extracts of soybean root nodules have been exposed to various gases and the spectral changes observed. In the presence of N2 hemoglobin is oxidized to hemiglobin; the opposite reaction is observed with H2. The extracts slowly reduce added mammalian cytochrome c and catalyze a slight deuterium-exchange reaction. In addition to their significance for the question of whether a hydrogenase exists in the nodule, these data support the suggestion that hemoglobin may act as an oxido-reduction catalyst in symbiotic nitrogen fixation. The hydrogenase of Azotobacter vinelandii strain O will reduce compounds in this organism whose absorption peaks correspond to flavins and cytochromes a.2, a.1, b and c. N2 appears specifically to oxidize flavins and the cytochrome b component.This publication has 7 references indexed in Scilit:
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