Assay Characteristics of Tannate Complexes of Human Pituitary Gonadotropin

Abstract
Tannate complexes of human pituitary gonadotropin (HPG) of urinary origin were assayed by the ventral prostate weight (VPW) assay and by the ovarian ascorbic acid depletion (OAAD) assay. Assays were also performed by injecting single doses of tannated and untannated HPG and determining the magnitude of the response at various times afterward. It was found that tannation increased the potency of HPG 2.4-fold in the VPW assay and was without effect in the OAAD assay. The results obtained with single-dose assays confirmed those obtained by conventional assays.