A Partial Order for Genes Determining Enzymes of the meta-Cleavage Pathway in Pseudomonas putida
- 1 May 1977
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 100 (1), 65-69
- https://doi.org/10.1099/00221287-100-1-65
Abstract
A partial order for genes which specify meta-cleavage pathway enzymes was derived from properties of a polarity mutant strain (PsU5) of P. putida NC1B10015 and a partial revertant, PsU5/R21. The polar mutation is within the 2-hydroxymuconic semialdehyde dehydrogenase gene and results in loss of detectable 2-hydroxymuconic semialdehyde hydrolase and 90-95% reduction in the activities of 4-oxalocrotonate tautomerase, 4-oxalocrotonate decarboxylase, 2-oxopent-4-enoate hydratase and 4-hydroxy-2-oxovalerate aldolase. The partial revertant PsU5/R21 regained wild-type levels of all enzymes except the aldehyde dehydrogenase. It is probable that all genes determining the dehydrogenase and subsequent enzymes are transcribed as a polycistronic message. This is the first report of mapping genes determining the enzymes of the meta-cleavage pathway.Keywords
This publication has 3 references indexed in Scilit:
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