A Simple Procedure for the Isolation of Brain Actin
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry & Biotechnology
- Vol. 11 (3), 351-360
- https://doi.org/10.1080/00327488108061774
Abstract
Actin has been isolated from bovine brain by forming an actin-containing gel from acetone powders. Solubilization of the gel and chromatography on Sephadex G150 produces actin in good yield. Acetone powders of crude pellets of brain homogenates obtained in the isolation of tubulin produces about 80% of the actin isolated from acetone powder of whole brain. The procedure therefore can be used to isolate both proteins from the same brain.This publication has 13 references indexed in Scilit:
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