malB region in Escherichia coli K-12: specialized transducing bacteriophages and first restriction map

Abstract

By starting from an E. coli K-12 strain with a .lambda. phage integrated in the malB region series of transducing phages carrying part or all of the malB region were isolated. Genetic mapping of the transduced malB fragments was accomplished by complementation and recombination with known mutations in the region. Using the DNA of these phages showed that the malB region is cleaved by the restriction enzymes BglII, EcoRI, HaeII, HincII, SalI and SstI, but not BamHI, HindIII, KpnI, PstI, XbaI or XhoI. A physical map was constructed and tentatively correlated with the genetic map.