Gene shuttling: moving of cloned DNA into and out of eukaryotic cells
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (4), 1243-1256
- https://doi.org/10.1093/nar/10.4.1243
Abstract
Successful shuttling of cloned DNA in eukaryotic cells should allow isolation of expressed genes. We tested the utility of cosmids for moving DNA into and out of eukaryotic cells. The unique cleavage of DNA at the cos site by the terminase function of lambda was exploited to maintain the linkage between the vector and inserted gene sequences, a prerequisite for successful rescue of the transforming DNA from high molecular weight DNA of the eukaryotic transformant. A cosmid recombinant containing the HSV thymidine kinase gene and a lambda recombinant containing the chicken thymidine kinase gene were used to test the feasability of this method. It was found that these recombinants can be rescued with high efficiency from DNA of HAT-resistant cells.Keywords
This publication has 16 references indexed in Scilit:
- DNA sequence of a mutation in the leader region of the yeast iso-1-cytochrome c mRNACell, 1981
- A small cosmid for efficient cloning of large DNA fragmentsGene, 1980
- Characteristics of an SV40-plasmid recombinant and its movement into and out of the genome of a murine cellCell, 1980
- Isolation of the chicken thymidine kinase gene by plasmid rescueNature, 1980
- Packaging of plasmid DNA containing the cohesive ends of coliphage lambdaGene, 1980
- Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage λ: “cosmids”Gene, 1978
- Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donorCell, 1978
- Packaging of ColE1 DNA having a lambda phage cohesive end siteMolecular Genetics and Genomics, 1978
- A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genomeCell, 1976
- Polarized packaging of bacteriophage lambda chromosomesJournal of Molecular Biology, 1975