Regulation of arachidonate‐mobilizing phospholipase A2 by phosphorylation via protein kinase C in macrophages
- 26 October 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 311 (3), 299-301
- https://doi.org/10.1016/0014-5793(92)81124-5
Abstract
Stimulation of 32P-labeled macrophages with phorbol ester caused an increase in phosphorylation of the intracellular, high molecular weight phospholipase A2. This increase in phosphorylation was accompanied by an increase in enzyme activity, but led to no detectable shift in the concentration dependence for Ca2+-induced activation. The phosphorylated phospholipase A2 could be dephosphorylated by treatment with acid phosphatase, and such treatment also reduced its catalytic activity. Together with previous data, these results indicate that the arachidonate-mobilizing phospholipase A2 is dually regulated by Ca2'+ (membrane interaction) and by phosphorylation (catalytic activity).Keywords
This publication has 20 references indexed in Scilit:
- Macrophage arachidonate-mobilizing phospholipase A2: Role of Ca2+ for membrane binding but not for catalytic activityBiochemical and Biophysical Research Communications, 1992
- An 100‐kDa arachidonate‐mobilizing phospholipase A2 in mouse spleen and the macrophage cell line J774European Journal of Biochemistry, 1991
- A novel arachidonic acid-selective cytosolic PLA2 contains a Ca2+-dependent translocation domain with homology to PKC and GAPCell, 1991
- Purification of a 110-kilodalton cytosolic phospholipase A2 from the human monocytic cell line U937.Proceedings of the National Academy of Sciences, 1990
- A phospholipase A2 hydrolyzing arachidonoyl‐phospholipids in mouse peritonea macrophagesFEBS Letters, 1989
- Properties and purification of an arachidonoyl-hydrolyzing phospholipase A2 from a macrophage cell line, RAW 264.7Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1988
- Calcium dependency of prostaglandin E2 production in rat glomerular mesangial cells. Evidence that protein kinase C modulates the Ca2+-dependent activation of phospholipase A2.JCI Insight, 1988
- Effect of cellular fatty acid composition on the phospholipase A2 activity of bone marrow-derived macrophages, and their ability to induce lucigenin-dependent chemiluminescenceBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1986
- Evidence for a catalytic role of phospholipase A in phorbol diester- and zymosan-induced mobilization of arachidonic acid in mouse peritoneal macrophagesBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970