Rapid detection of intercellular adhesion molecule 1 (G241R and K469E) polymorphisms by a novel PCR?SSP assay
- 1 April 2007
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 69 (4), 338-341
- https://doi.org/10.1111/j.1399-0039.2007.00813.x
Abstract
Intercellular adhesion molecule 1 (ICAM-1) is a cell surface glycoprotein member of the immunoglobulin superfamily and is actively involved in immune and inflammatory responses. We introduce a novel polymerase chain reaction-sequence-specific primers (PCR-SSP) method for rapid and simultaneous genotyping of ICAM-1 G241R and K469E polymorphisms. In a total of 184 DNA samples that have been previously analyzed for these polymorphisms using polymerase chain reaction-restriction fragment length polymorphism technique, re-genotyping of all samples with this new assay showed accurate and reproducible results. As PCR-SSP-based genotyping protocols are more convenient and cost-effective to do, it could therefore offer a valuable tool for assessment of ICAM-1 polymorphisms to which more confirmatory studies are needed.Keywords
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