Über die Senkung der Körpertemperatur durch Adenylthiomethylpentose

Abstract

Adenylthiomethylpentose, C11H15O3N5S, has been isolated from yeast as a crystalline cornpd. Adult guinea pigs, 500-800 g. in wt., were given by mouth 0.2 g. per kg. body wt. of adenylthiomethylpentose and related compounds and the rectal temp. recorded. Within about 1 hr., adenylthiomethylpentose and its hydrochloride caused a fall in body temp. of about 1[degree]. The temp. returned to normal in about 24 hrs. Hypoxan-thylthiomethylpentose caused a similar but weaker effect. Adenosine, muscle and yeast adenylic acids, cozymase, thio-methylpentose, thiomethylpentite, an equimolar mixture of adenine sulfate and thiopentose, thiomethylacetaldehydedi-ethylacetal, thiomethylacetaldehyde, homocystme and methionine had no effect on body temp. The adenylthiomethylpentose hydrochloride, m. 161[degree]-162[degree], was prepared by dissolving the pentose in the calculated amt. of N HC1, concentrating the soln. in vacuo, and recrystallizing the product from 95% ethyl alcohol. Hypoxanthylthiomethylpentose was prepared by suspending 5 g. adenylthiomethylpentose in 150 cc. H2O, adding 3 g. NaNO2, and heating the mixture to 80[degree]. Ten cc. of acetic acid were added, the soln. evaporated to dryness in vacuo, and the residue shaken for 12 hrs. with 20 cc. pyridine and 3 cc. acetic anhydride. The mixture was evaporated to dryness, the residue dissolved in 70 cc. H2O, and the acetylhypoxanthylthiomethylpentose extracted with chloroform. The acetyl compound was dissolved in 20 cc. methyl alcohol, which is saturated with NH8, and after 24 hrs., crystals of hypoxanthylthiomethylpentose (C11H14O4N4S), m. 220.5[degree]-221.5[degree], were obtained.