High-Efficiency Fluorescence Quenching of Conjugated Polymers by Proteins
- 27 April 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 124 (20), 5642-5643
- https://doi.org/10.1021/ja025899u
Abstract
The fluorescence of the water-soluble anionic conjugated polymer, poly[lithium 5-methoxy-2-(4-sulfobutoxy)-1,4-phenylenevinylene] (MBL-PPV), is quenched in dilute aqueous solution by cytochrome c, a small, naturally occurring electron-transfer protein. The large value obtained for the Stern−Volmer constant (Ksv = 3.2 × 108 at pH 7.4, and ∼109 in acidic solutions) is attributed to a combination of two factors: (1) facile ET between the luminescent semiconducting polymer and the protein and (2) the Columb attraction between the oppositely charged polyelectrolytes. This system shows significant potential for biosensor applications.Keywords
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