Isolation of endonuclease from mammalian cells by DNA - cellulose chromatograpby
Open Access
- 1 June 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (6), 905-914
- https://doi.org/10.1093/nar/2.6.905
Abstract
By means of DNA-cellulose chromatography an enzyme with endonucleolytlc activity has been Isolated from nuclear acidic protein fraction of mammalian cells. The main active fraction, Bluted at 0.7 M NaCl, affects the velocity sedimentatian of UV-irradiated and alkylated DNA, resulting in a decrease of the molecular weight. The fraction is completely Inactive using native as well as heat-denatured DNA.Keywords
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