Temporal encoding of movement kinematics in the discharge of primate primary motor and premotor neurons

Abstract
1. Several neurophysiological studies of the primary motor and premotor cortices have shown that the movement parameters direction, distance, and target position are correlated with the discharge of single neurons. Here we investigate whether the correlations with these parameters occur simultaneously (i.e., parallel processing), or sequentially (i.e., serial processing). 2. The single-unit data used for the analyses presented in this paper are the same as those used in our earlier study of neuronal specification of movement parameters. We recorded the activity of single neurons in the primary motor and premotor cortices of two rhesus monkeys (Macaca mulatta) while the animals performed reaching movements made in a horizontal plane. Specifically, the animals moved from a centrally located start position to 1 of 48 targets (1 cm2) placed at eight different directions (0-360 degrees in 45 degrees intervals) and six distances (1.4-5.4 cm in 0.8-cm increments) from the start position. 3. We analyzed 130 task-related cells; of these, 127 (99 in primary motor cortex, 28 near the superior precentral sulcus) had average discharges that were significantly modulated with the movement and were related to movement direction, distance, or target position. To determine the temporal profile of the correlation of each cell's discharge with the three parameters, we performed a regression analysis of the neural discharge. We calculated partial R2s for each parameter and the total R2 for the model as a function of time. 4. The discharge of the majority of units (73.2%) was significantly correlated for some time with all three parameters. Other units were found that correlated with different combinations of pairs of parameters (21.3%), and a small number of units appeared to code for only one parameter (5.5%). There was no obvious difference in the presence of correlations between cells recorded in the primary motor versus premotor cortices. 5. On average we found a clear temporal segregation and ordering in the onset of the parameter-related partial R2 values: direction-related discharge occurred first (115 ms before movement onset), followed sequentially by target position (57 ms after movement onset) and movement distance (248 ms after movement onset). Some overlap in the timing of the correlation of these parameters was evident. We found a similar sequential ordering for the latency of the peak of the R2 curves (48, 254, and 515 ms after movement onset, respectively, for direction, target position, and distance).(ABSTRACT TRUNCATED AT 400 WORDS)