Action of cytochalasin D on cells of established lines. III. Zeiosis and movements at the cell surface.
Open Access
- 1 March 1975
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 64 (3), 644-667
- https://doi.org/10.1083/jcb.64.3.644
Abstract
The projection of knobby protuberances at the cell surface (zeiosis) is a general cellular response to cytochalasin D (CD), resulting from herniation of endoplasm through undefended places of the cortex during cell contractions and displacement of microfilaments induced by CD. Zeiosis is prevented by agents that interfere with the contractile response to CD, such as inhibitors of energy metabolism or cyclic AMP. The developed protrusions, which remain relatively stable in the presence of CD, contain chiefly mono- or subribosomes, and occasionally other organelles normally resident in endoplasm; compact microfilament felt occupies their bases and extends into their proximal stalks. Protein synthesis in the knobs is less than half of that in the polyribosome-containing endoplasm residual in the main body of the cell. Knobs first protrude singly near the margin of the contracting cells and rapidly cluster into small groups in the periphery even at lower temperature. The clusters then migrate centripetally and coalesce into a large aggregate near the apex of the immobilized and retracted cell: this movement is energy- and temperature-dependent. Aggregation is more prominent and stable in cell lines of epithelial derivation than in fibroblastic or other lines in which nuclear extrusion occurs more readily. The latter is regarded as a special manifestation of zeiosis. Macromarkers, such as latex spherules, migrate like the zeiotic knobs on the cell surfaces in the presence of CD. The aggregated knobs, although persistent for days in the presence of CD, are rapidly recessed after withdrawal of the agent as ruffling is resumed and the cells spread. These movements are discussed in terms of current concepts of mobility of the cell membrane.Keywords
This publication has 52 references indexed in Scilit:
- Properties of particle movement in the plasma membrane of 3T3 mouse fibroblastsExperimental Cell Research, 1974
- A quantitative difference in the movement of marker particles in the plasma membrane of 3T3 mouse fibroblasts and their polyoma transformantsExperimental Cell Research, 1973
- A quantitation of movement of marker particles in the plasma membrane of 3T3 mouse fibroblasts: Experimental methodsExperimental Cell Research, 1973
- CYTOCHALASIN BThe Journal of cell biology, 1973
- CYTOCHALASIN B AND THE ADENOSINE TRIPHOSPHATE CONTENT OF TREATED FIBROBLASTSThe Journal of cell biology, 1972
- Microfilaments in Cellular and Developmental ProcessesScience, 1971
- The locomotion of fibroblasts in culture: III. Movements of particles on the dorsal surface of the leading lamellaExperimental Cell Research, 1970
- The locomotion of fibroblasts in cultureExperimental Cell Research, 1970
- Mitosis in cultures of newt tissuesExperimental Cell Research, 1955
- Cultivation of neurons from the adult human cerebral and cerebellar cortexJournal of Anatomy, 1951