Identification of urinary metabolites of 2,4-dinitrotoluene (2,4-DNT) in rats.

Abstract

Metabolites of 2,4-dinitrotoluene (2,4-DNT) in urine were detected by TLC after repeated oral administration to male Wistar rats. These metabolites, 2-amino-4-nitrotoluene, 4-amino-2-nitrotoluene, 2,4-diaminotoluene, 2,4-dinitrobenzyl alcohol, 2-amino-4-nitrobenzyl alcohol, 4-amino-2-nitrobenzyl alcohol, 2-nitro-4-acetylaminotoluene, 2-amino-4-acetylaminotoluene and 2-amino-4-acetylaminobenzoic acid, were identified by comparison of UV and mass spectral data with those of authentic samples. The probable metabolism of 2,4-DNT in rats was discussed.