Abstract
Parallel tests with aphid-transfer techniques and enzyme-linked immunosorbent assay (ELISA) indicated that ELISA was sensitive enough to detect barley yellow dwarf virus in oat [Avena byzantina ''Coast Black''] leaf extracts. The reaction was specific enough to distinguish easily RPV and MAV, 2 distinctive vector-specific isolates of the virus. A 3rd type of isolate, PAV, sometimes reacted, usually weakly, in heterologous tests with MAV antiserum. ELISA apparently has potential for simplifying survey and assay work with this phloem-restricted luteovirus.