A Soluble Mitochondrial ATP Synthetase Complex Catalyzing ATP-Phosphate and ATP-ADP Exchange
- 1 September 1971
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 68 (9), 2181-2184
- https://doi.org/10.1073/pnas.68.9.2181
Abstract
The highly purified soluble ATP synthetase complex from mitochondria, containing energy-transfer Factor A (the terminal ADP phosphorylation enzyme of oxidative phosphorylation) and Factor D, catalyzes ATP-P(i) and ATP-ADP exchange reactions. The ATP-P(i) exchange activity is inhibited by low concentrations of the uncouplers of oxidative phosphorylation, oligomycin and p-chloromercnriphenylsulfonate. It is stimulated threefold by dithiothreitol and is Mg(++) dependent. Antiserum to coupling factor 1 (F(1)) also inhibits the ATP-P(i) exchange. The ATP-ADP exchange activity appears to be greater than the ATP-P(i) exchange activity. The results suggest that the nonphosphorylated high-energy intermediate (X approximately C), and possibly the phosphorylated intermediate (X approximately P), are formed on the synthetase. Sites of uncoupler and oligomycin action reside in the terminal ATP synthetase.Keywords
This publication has 18 references indexed in Scilit:
- Detection of a phosphorylated intermediate in mitochondrial oxidative phosphorylationBiochemical and Biophysical Research Communications, 1970
- Oxidative Phosphorylation in MitochondriaAnnual Review of Biochemistry, 1969
- The role of Factor B in the energy transfer reactions of oxidative phosphorylation.Proceedings of the National Academy of Sciences, 1968
- Molecular weight, sulfhydryl conten, and phosphorylation of a homogeneous mitochondrial nucleoside diphosphokinase.1968
- Conformational basis of energy transformations in membrane systemsArchives of Biochemistry and Biophysics, 1968
- CHEMIOSMOTIC COUPLING IN OXIDATIVE AND PHOTOSYNTHETIC PHOSPHORYLATIONBiological Reviews, 1966
- Coupling of Phosphorylation to Electron and Hydrogen Transfer by a Chemi-Osmotic type of MechanismNature, 1961
- The Adenosine Triphosphate-Adenosine Diphosphate Exchange Reaction of Oxidative PhosphorylationJournal of Biological Chemistry, 1958
- OXIDATIVE PHOSPHORYLATION BY AN ENZYME COMPLEX FROM EXTRACTS OF MITOCHONDRIA .3. SPAN CYTOCHROME-C TO OXYGEN1956
- Mechanism of Phosphorylation in the Respiratory ChainNature, 1953